Upconverting nanoparticles optimized for long-term tracking of single molecules!

Publication

UpConverting NanoParticles (UCNPs) are capable of emitting visible light very efficiently after excitation by infrared light. UCNPs’ Anti-Stockes emission has been used for a wide range of applications. In the medical field, their spectrum of applications ranges from biosensors and bioimaging to photodynamic cancer treatments and targeted drug delivery systems.

Frédéric Przybilla, from Yves Mély's team Biophotonics of the molecular and cellular interactions, has demonstrated the superiority of these UCNP markers for super-resolution microscopy applications.Developments during this project have brought UCNP technology to maturity for long-term single molecule tracking (SMT) applications.

 By adapting the targeting agent, this innovation opens up a very wide field of new applications in medical biotechnology.

 

Oleksii Dukhno, Srijayee Ghosh, Vanille Greiner, Sophie Bou, Julien Godet, Verena Muhr, Markus Buchner, Thomas Hirsch, Yves Mély, Frédéric Przybilla. Targeted Single Particle Tracking with Upconverting Nanoparticles. ACS Appl. Mater. Interfaces 2024, 16, 9, 11217–11227

doi.org/10.1021/acsami.3c17116

Schematic representation of the UCNP-IgE marker used to track FcεRI receptors on the surface of living RBL-2H3 cells. Bright-field image of the cells studied, region of interest showing anti-Stokes emission from UCNPs, trajectories obtained after video analysis.
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