Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues
Isomorphic and expanded fluorescent nucleoside analogues are artificial mimics of the natural non-emissive nucleosides, resembling them by their molecular size, geometry, and the presence of H-bonding groups. At the same time, their fluorescence enables many applications in biophysics and biochemistry. In our recent review published in Chem. Soc. Rev. we survey the fundamental photophysical phenomena that underlie the environment sensitivity of such fluorophores and show how the knowledge of these mechanisms can be utilized in fluorescence-based studies of nucleic acids and their interactions.
Reference: Fundamental photophysics of isomorphic and expanded fluorescent nucleoside analogues. Dziuba D, Didier P, Ciaco S, Barth A, Seidel CAM, Mély Y.
Chem Soc Rev. 2021 Jun 21;50(12):7062-7107. DOI: 10.1039/d1cs00194a
